Latest Posts by smparticle2 - Page 3

Sixty Symbols has a great new video explaining the laboratory set-up for demoing a Kelvin-Helmholtz instability. You can see a close-up from the demo above. Here the pink liquid is fresh water and the blue is slightly denser salt water. When the tank holding them is tipped, the lighter fresh water flows upward while the salt water flows down. This creates a big velocity gradient and lots of shear at the interface between them. The situation is unstable, meaning that any slight waviness that forms between the two layers will grow (exponentially, in this case). Note that for several long seconds, it seems like nothing is happening. That’s when any perturbations in the system are too small for us to see. But because the instability causes those perturbations to grow at an exponential rate, we see the interface go from a slight waviness to a complete mess in only a couple of seconds. The Kelvin-Helmholtz instability is incredibly common in nature, appearing in clouds, ocean waves, other planets’ atmospheres, and even in galaxy clusters! (Image and video credit: Sixty Symbols)

You are the center of wonderland & Keep the last glow in mind by Jana Luo

There is a time when it is necessary to abandon the used clothes, which already have the shape of our body and to forget our paths, which takes us always to the same places. This is the time to cross the river: and if we don’t dare to do it, we will have stayed, forever beneath ourselves

Fernando Pessoa (via paizleyrayz)

Green method developed for making artificial spider silk

A team of architects and chemists from the University of Cambridge has designed super-stretchy and strong fibres which are almost entirely composed of water, and could be used to make textiles, sensors and other materials. The fibres, which resemble miniature bungee cords as they can absorb large amounts of energy, are sustainable, non-toxic and can be made at room temperature.

This new method not only improves upon earlier methods of making synthetic spider silk, since it does not require high energy procedures or extensive use of harmful solvents, but it could substantially improve methods of making synthetic fibres of all kinds, since other types of synthetic fibres also rely on high-energy, toxic methods. The results are reported in the journal Proceedings of the National Academy of Sciences.

Spider silk is one of nature’s strongest materials, and scientists have been attempting to mimic its properties for a range of applications, with varying degrees of success. “We have yet to fully recreate the elegance with which spiders spin silk,” said co-author Dr Darshil Shah from Cambridge’s Department of Architecture.

Read more.

How do you build a metal nanoparticle?

Novel theory explains how metal nanoparticles form

Although scientists have for decades been able to synthesize nanoparticles in the lab, the process is mostly trial and error, and how the formation actually takes place is obscure. However, a study recently published in Nature Communications by chemical engineers at the University of Pittsburgh’s Swanson School of Engineering explains how metal nanoparticles form.

“Thermodynamic Stability of Ligand-Protected Metal Nanoclusters” (DOI: 10.1038/ncomms15988) was co-authored by Giannis Mpourmpakis, assistant professor of chemical and petroleum engineering, and PhD candidate Michael G. Taylor. The research, completed in Mpourmpakis’ Computer-Aided Nano and Energy Lab (C.A.N.E.LA.), is funded through a National Science Foundation CAREER award and bridges previous research focused on designing nanoparticles for catalytic applications.

“Even though there is extensive research into metal nanoparticle synthesis, there really isn’t a rational explanation why a nanoparticle is formed,” Dr. Mpourmpakis said. “We wanted to investigate not just the catalytic applications of nanoparticles, but to make a step further and understand nanoparticle stability and formation. This new thermodynamic stability theory explains why ligand-protected metal nanoclusters are stabilized at specific sizes.”

Read more.

“I always wanted to be a mental health therapist.  Ever since high school, I’ve enjoyed encouraging people and giving them hope.  But I lost my way.  I got caught in a world of addiction.  I lost ten years of my life to drugs.  I stopped when I became pregnant with my child, but by that time it was too late to go back to school.  I started working as an office manager.  I never completely lost my dream.  But I just put it on a shelf for thirty years.  Then five years ago I to…ok it off the shelf.  I heard a lady in my choir talking about how she enrolled in community college.  I drove there the very next day.  I was so nervous when I filled out the application.  I was so nervous the first day of class.  All the old voices were telling me: ‘You never finish anything.’  But I said ‘fuck you’ to the old voices.  And I started getting A’s.  On my first test, I got the only perfect score in the class.  I graduated at the age of 50.  I got my Masters at 55.  And just last night I completed a mental health first aid course.  I’m so close now.  There’s still fear there.  I used to be afraid of it never happening.  Now I’m afraid of it happening.  The old voices try to come back sometimes.  They tell me: ‘You can rest,’ or ‘You’ve earned a break.’  But I’m not stopping this time.  Somebody out there is waiting for me to finish because they need my help.“

Big Improvements to Brain-Computer Interface

When people suffer spinal cord injuries and lose mobility in their limbs, it’s a neural signal processing problem. The brain can still send clear electrical impulses and the limbs can still receive them, but the signal gets lost in the damaged spinal cord.

The Center for Sensorimotor Neural Engineering (CSNE)—a collaboration of San Diego State University with the University of Washington (UW) and the Massachusetts Institute of Technology (MIT)—is working on an implantable brain chip that can record neural electrical signals and transmit them to receivers in the limb, bypassing the damage and restoring movement. Recently, these researchers described in a study published in the journal Nature Scientific Reports a critical improvement to the technology that could make it more durable, last longer in the body and transmit clearer, stronger signals.

The technology, known as a brain-computer interface, records and transmits signals through electrodes, which are tiny pieces of material that read signals from brain chemicals known as neurotransmitters. By recording brain signals at the moment a person intends to make some movement, the interface learns the relevant electrical signal pattern and can transmit that pattern to the limb’s nerves, or even to a prosthetic limb, restoring mobility and motor function.

The current state-of-the-art material for electrodes in these devices is thin-film platinum. The problem is that these electrodes can fracture and fall apart over time, said one of the study’s lead investigators, Sam Kassegne, deputy director for the CSNE at SDSU and a professor in the mechanical engineering department.

Kassegne and colleagues developed electrodes made out of glassy carbon, a form of carbon. This material is about 10 times smoother than granular thin-film platinum, meaning it corrodes less easily under electrical stimulation and lasts much longer than platinum or other metal electrodes.

“Glassy carbon is much more promising for reading signals directly from neurotransmitters,” Kassegne said. “You get about twice as much signal-to-noise. It’s a much clearer signal and easier to interpret.”

The glassy carbon electrodes are fabricated here on campus. The process involves patterning a liquid polymer into the correct shape, then heating it to 1000 degrees Celsius, causing it become glassy and electrically conductive. Once the electrodes are cooked and cooled, they are incorporated into chips that read and transmit signals from the brain and to the nerves.

Researchers in Kassegne’s lab are using these new and improved brain-computer interfaces to record neural signals both along the brain’s cortical surface and from inside the brain at the same time.

“If you record from deeper in the brain, you can record from single neurons,” said Elisa Castagnola, one of the researchers. “On the surface, you can record from clusters. This combination gives you a better understanding of the complex nature of brain signaling.”

A doctoral graduate student in Kassegne’s lab, Mieko Hirabayashi, is exploring a slightly different application of this technology. She’s working with rats to find out whether precisely calibrated electrical stimulation can cause new neural growth within the spinal cord. The hope is that this stimulation could encourage new neural cells to grow and replace damaged spinal cord tissue in humans. The new glassy carbon electrodes will allow her to stimulate, read the electrical signals of and detect the presence of neurotransmitters in the spinal cord better than ever before.

What’s Up for July 2017

Prepare for the August total solar eclipse by observing the moon phases this month. Plus, two meteor showers peak at the end of July.

Solar eclipses occur when the new moon passes between the Earth and the sun and moon casts a traveling shadow on Earth. A total solar eclipse occurs when the new moon is in just the right position to completely cover the sun’s disk.

This will happen next month on August 21, when the new month completely blocks our view of the sun along a narrow path from Oregon to South Carolina.

It may even be dark enough during the eclipse to see some of the brighter stars and few planets!

Two weeks before or after a solar eclipse, there is often, but not always, a lunar eclipse. This happens because the full moon, the Earth and the sun will be lined up with Earth in the middle.

Beginning July 1, we can see all the moon’s phases.

Many of the Apollo landing sites are on the lit side of the first quarter moon. But to see these sites, you’ll have to rely on images for lunar orbiting spacecraft.

On July 9, the full moon rises at sunset and July 16 is the last quarter. The new moon begins on July 23 and is the phase we’ll look forward to in August, when it will give us the total solar eclipse. The month of July ends with a first quarter moon.

We’ll also have two meteor showers, both of which peak on July 30. The Delta Aquarids will have 25 meteors per hour between midnight and dawn.

The nearby slow and bright Alpha Capricornids per at 5 per hour and often produce fireballs.

Watch the full video:

Make sure to follow us on Tumblr for your regular dose of space: http://nasa.tumblr.com

New discovery could be a major advance for understanding neurological diseases

The discovery of a new mechanism that controls the way nerve cells in the brain communicate with each other to regulate our learning and long-term memory could have major benefits to understanding how the brain works and what goes wrong in neurodegenerative disorders such as epilepsy and dementia. The breakthrough, published in Nature Neuroscience, was made by scientists at the University of Bristol and the University of Central Lancashire.   The findings will have far-reaching implications in many aspects of neuroscience and understanding how the brain works.

The human brain contains around 100-billion nerve cells, each of which makes about 10,000 connections to other cells, called synapses. Synapses are constantly transmitting information to, and receiving information from other nerve cells. A process, called long-term potentiation (LTP), increases the strength of information flow across synapses. Lots of synapses communicating between different nerve cells form networks and LTP intensifies the connectivity of the cells in the network to make information transfer more efficient. This LTP mechanism is how the brain operates at the cellular level to allow us to learn and remember. However, when these processes go wrong they can lead to neurological and neurodegenerative disorders.

Precisely how LTP is initiated is a major question in neuroscience. Traditional LTP is regulated by the activation of special proteins at synapses called NMDA receptors. This study, by Professor Jeremy Henley and co-workers reports a new type of LTP that is controlled by kainate receptors.

This is an important advance as it highlights the flexibility in the way synapses are controlled and nerve cells communicate. This, in turn, raises the possibility of targeting this new pathway to develop therapeutic strategies for diseases like dementia, in which there is too little synaptic transmission and LTP, and epilepsy where there is too much inappropriate synaptic transmission and LTP.

Jeremy Henley, Professor of Molecular Neuroscience in the University’s School of Biochemistry in the Faculty of Biomedical Sciences, said: “These discoveries represent a significant advance and will have far-reaching implications for the understanding of memory, cognition, developmental plasticity and neuronal network formation and stabilisation. In summary, we believe that this is a groundbreaking study that opens new lines of inquiry which will increase understanding of the molecular details of synaptic function in health and disease.”

Dr Milos Petrovic, co-author of the study and Reader in Neuroscience at the University of Central Lancashire added: “Untangling the interactions between the signal receptors in the brain not only tells us more about the inner workings of a healthy brain, but also provides a practical insight into what happens when we form new memories. If we can preserve these signals it may help protect against brain diseases.

“This is certainly an extremely exciting discovery and something that could potentially impact the global population. We have discovered potential new drug targets that could help to cure the devastating consequences of dementias, such as Alzheimer’s disease. Collaborating with researchers across the world in order to identify new ways to fight disease like this is what world-class scientific research is all about, and we look forward to continuing our work in this area.”

Scientists report the first scientific results from NASA’s Juno mission, now in orbit around Jupiter.

This week, a suite of 46 separate scientific papers describe different aspects of the giant planet Jupiter, from its massive polar cyclones, to its complex magnetic field, to its unique radiation environment. The papers mark the first full scientific results from NASA’s Juno mission, which arrived in orbit around Jupiter last summer. Later this July, the craft is slated to overfly the planet’s Great Red Spot, bringing back still more data. Juno program scientist Jared Espley and Juno radiation monitoring investigation lead Heidi Becker join Ira to sum up some of the Jovian surprises, as well as give a preview of what still lies ahead for the Juno mission. Listen here to learn more.

[Photos by NASA/JPL/MSSS/Gerald Eichstädt/Justin Cowart/Alexis Tranchandon/Solaris]

↳ Whisper of the Heart ||

Grading a slew of mediocre final papers, the grad student watches his months of arduous teaching bear little fruit.

May 18, 1969 — Inside mission control at the Johnson Space Center, Houston, during the first day of the Apollo 10 mission. (NASA)

Faster, smaller, more powerful computer chips: Hafnia dons a new face

Materials research creates potential for improved computer chips and transistors

It’s a material world, and an extremely versatile one at that, considering its most basic building blocks – atoms – can be connected together to form different structures that retain the same composition.

Diamond and graphite, for example, are but two of the many polymorphs of carbon, meaning that both have the same chemical composition and differ only in the manner in which their atoms are connected. But what a world of difference that connectivity makes: The former goes into a ring and costs thousands of dollars, while the latter has to sit content within a humble pencil.

The inorganic compound hafnium dioxide commonly used in optical coatings likewise has several polymorphs, including a tetragonal form with highly attractive properties for computer chips and other optical elements. However, because this form is stable only at temperatures above 3100 degrees Fahrenheit – think blazing inferno – scientists have had to make do with its more limited monoclinic polymorph. Until now.

Read more.

(Image caption: New model mimics the connectivity of the brain by connecting three distinct brain regions on a chip. Credit: Disease Biophysics Group/Harvard University)

Multiregional brain on a chip

Harvard University researchers have developed a multiregional brain-on-a-chip that models the connectivity between three distinct regions of the brain. The in vitro model was used to extensively characterize the differences between neurons from different regions of the brain and to mimic the system’s connectivity.

The research was published in the Journal of Neurophysiology.

“The brain is so much more than individual neurons,” said Ben Maoz, co-first author of the paper and postdoctoral fellow in the Disease Biophysics Group in the Harvard John A. Paulson School of Engineering and Applied Sciences (SEAS). “It’s about the different types of cells and the connectivity between different regions of the brain. When modeling the brain, you need to be able to recapitulate that connectivity because there are many different diseases that attack those connections.”

“Roughly twenty-six percent of the US healthcare budget is spent on neurological and psychiatric disorders,” said Kit Parker, the Tarr Family Professor of Bioengineering and Applied Physics Building at SEAS and Core Faculty Member of the Wyss Institute for Biologically Inspired Engineering at Harvard University. “Tools to support the development of therapeutics to alleviate the suffering of these patients is not only the human thing to do, it is the best means of reducing this cost.“

Researchers from the Disease Biophysics Group at SEAS and the Wyss Institute modeled three regions of the brain most affected by schizophrenia  — the amygdala, hippocampus and prefrontal cortex.

They began by characterizing the cell composition, protein expression, metabolism, and electrical activity of neurons from each region in vitro.

“It’s no surprise that neurons in distinct regions of the brain are different but it is surprising just how different they are,” said Stephanie Dauth, co-first author of the paper and former postdoctoral fellow in the Disease Biophysics Group. “We found that the cell-type ratio, the metabolism, the protein expression and the electrical activity all differ between regions in vitro. This shows that it does make a difference which brain region’s neurons you’re working with.”

Next, the team looked at how these neurons change when they’re communicating with one another. To do that, they cultured cells from each region independently and then let the cells establish connections via guided pathways embedded in the chip.

The researchers then measured cell composition and electrical activity again and found that the cells dramatically changed when they were in contact with neurons from different regions.

“When the cells are communicating with other regions, the cellular composition of the culture changes, the electrophysiology changes, all these inherent properties of the neurons change,” said Maoz. “This shows how important it is to implement different brain regions into in vitro models, especially when studying how neurological diseases impact connected regions of the brain.”

To demonstrate the chip’s efficacy in modeling disease, the team doped different regions of the brain with the drug Phencyclidine hydrochloride — commonly known as PCP — which simulates schizophrenia. The brain-on-a-chip allowed the researchers for the first time to look at both the drug’s impact on the individual regions as well as its downstream effect on the interconnected regions in vitro.

The brain-on-a-chip could be useful for studying any number of neurological and psychiatric diseases, including drug addiction, post traumatic stress disorder, and traumatic brain injury.

"To date, the Connectome project has not recognized all of the networks in the brain,” said Parker. “In our studies, we are showing that the extracellular matrix network is an important part of distinguishing different brain regions and that, subsequently, physiological and pathophysiological processes in these brain regions are unique. This advance will not only enable the development of therapeutics, but fundamental insights as to how we think, feel, and survive.”

Good vibrations no longer needed for speakers as research encourages graphene to talk

A pioneering new technique that encourages the wonder material graphene to “talk” could revolutionise the global audio and telecommunications industries.

Researchers from the University of Exeter have devised a ground-breaking method to use graphene to generate complex and controllable sound signals. In essence, it combines speaker, amplifier and graphic equaliser into a chip the size of a thumbnail.

Traditional speakers mechanically vibrate to produce sound, with a moving coil or membrane pushing the air around it back and forth. It is a bulky technology that has hardly changed in more than a century.

This innovative new technique involves no moving parts. A layer of the atomically thin material graphene is rapidly heated and cooled by an alternating electric current, and transfer of this thermal variation to the air causes it to expand and contract, thereby generating sound waves.

Read more.

Golden Gate Bridge by Jason Jko

Howl’s Moving Castle, 2004

This car race involved years of training, feats of engineering, high-profile sponsorships, competitors from around the world and a racetrack made of gold.

But the high-octane competition, described as a cross between physics and motor-sports, is invisible to the naked eye. In fact, the track itself is only a fraction of the width of a human hair, and the cars themselves are each comprised of a single molecule.

The Nanocar Race, which happened over the weekend at Le centre national de la recherché scientific in Toulouse, France, was billed as the “first-ever race of molecule-cars.”

It’s meant to generate excitement about molecular machines. Research on the tiny structures won last year’s Nobel Prize in Chemistry, and they have been lauded as the “first steps into a new world,” as The Two-Way reported.

Microscopic Cars Square Off In Big Race

Image: CNRS

Black phosphorus holds promise for the future of electronics

Discovered more than 100 years ago, black phosphorus was soon forgotten when there was no apparent use for it. In what may prove to be one of the great comeback stories of electrical engineering, it now stands to play a crucial role in the future of electronic and optoelectronic devices.

With a research team’s recent discovery, the material could possibly replace silicon as the primary material for electronics. The team’s research, led by Fengnian Xia, Yale’s Barton L. Weller Associate Professor in Engineering and Science, is published in the journal Nature Communications April 19.

With silicon as a semiconductor, the quest for ever-smaller electronic devices could soon reach its limit. With a thickness of just a few atomic layers, however, black phosphorus could usher in a new generation of smaller devices, flexible electronics, and faster transistors, say the researchers.

That’s due to two key properties. One is that black phosphorus has a higher mobility than silicon—that is, the speed at which it can carry an electrical charge. The other is that it has a bandgap, which gives a material the ability to act as a switch; it can turn on and off in the presence of an electric field and act as a semiconductor. Graphene, another material that has generated great interest in recent years, has a very high mobility, but it has no bandgap.

Read more.

Shortly after he finished filming on the opulent set of Baz Luhrmann’s ‘The Great Gatsby,’ Joel headed off to the Jordanian desert to begin training for 'Zero Dark Thirty.'  With a heavy dose of mock horror, he said that it was quite a shock to his delicate system:

“An experience like ‘Gatsby’ really spoils you because you are treated like a king. You’re given a big trailer, someone brings fresh flowers to your trailer, there are dates and walnuts and coconut water in your fridge … really living large.“

Then, when he suddenly found himself roughing it in the torrid desert, “sharing a cubicle with five other guys, half of them military, and carrying 50-60 kilos of equipment … It was like, 'Baz!  Come and save me!’  You get a reality check."   

First and last appearances.

How to Make a Motor Neuron

A team of scientists has uncovered details of the cellular mechanisms that control the direct programming of stem cells into motor neurons. The scientists analyzed changes that occur in the cells over the course of the reprogramming process. They discovered a dynamic, multi-step process in which multiple independent changes eventually converge to change the stem cells into motor neurons.

“There is a lot of interest in generating motor neurons to study basic developmental processes as well as human diseases like ALS and spinal muscular atrophy,” said Shaun Mahony, assistant professor of biochemistry and molecular biology at Penn State and one of the lead authors of the paper. “By detailing the mechanisms underlying the direct programing of motor neurons from stem cells, our study not only informs the study of motor neuron development and its associated diseases, but also informs our understanding of the direct programming process and may help with the development of techniques to generate other cell types.”

The direct programming technique could eventually be used to regenerate missing or damaged cells by converting other cell types into the missing one. The research findings, which appear online in the journal Cell Stem Cell on December 8, 2016, show the challenges facing current cell-replacement technology, but they also outline a potential pathway to the creation of more viable methods.

“Despite having a great therapeutic potential, direct programming is generally inefficient and doesn’t fully take into account molecular complexity,” said Esteban Mazzoni, an assistant professor in New York University’s Department of Biology and one of the lead authors of the study. “However, our findings point to possible new avenues for enhanced gene-therapy methods.”

The researchers had shown previously that they can transform mouse embryonic stem cells into motor neurons by expressing three transcription factors – genes that control the expression of other genes – in the stem cells. The transformation takes about two days. In order to better understand the cellular and genetic mechanisms responsible for the transformation, the researchers analyzed how the transcription factors bound to the genome, changes in gene expression, and modifications to chromatin at 6-hour intervals during the transformation.

“We have a very efficient system in which we can transform stem cells into motor neurons with something like a 90 to 95 percent success rate by adding the cocktail of transcription factors,” said Mahony. “Because of that efficiency, we were able to use our system to tease out the details of what actually happens in the cell during this transformation.”

“A cell in an embryo develops by passing through several intermediate stages,” noted Uwe Ohler, senior researcher at the Max Delbrück Center for Molecular Medicine (MDC) in Berlin and one of the lead authors of the work. “But in direct programming we don’t have that: we replace the gene transcription network of the cell with a completely new one at once, without the progression through intermediate stages. We asked, what are the timing and kinetics of chromatin changes and transcription events that directly lead to the final cell fate?“

The research team found surprising complexity – programming of these stem cells into neurons is the result of two independent transcriptional processes that eventually converge. Early on in the process, two of the transcription factors – Isl1 and Lhx3 – work in tandem, binding to the genome and beginning a cascade of events including changes to chromatin structure and gene expression in the cells. The third transcription factor, Ngn2, acts independently making additional changes to gene expression. Later in the transformation process, Isl1 and Lhx3 rely on changes in the cell initiated by Ngn2 to help complete the transformation. In order for direct programming to successfully achieve cellular conversion, it must coordinate the activity of the two processes.

“Many have found direct programming to be a potentially attractive method as it can be performed either in vitro – outside of a living organism – or in vivo – inside the body and, importantly, at the site of cellular damage,” said Mazzoni. “However, questions remain about its viability to repair cells – especially given the complex nature of the biological process. Looking ahead, we think it’s reasonable to use this newly gained knowledge to, for instance, manipulate cells in the spinal cord to replace the neurons required for voluntary movement that are destroyed by afflictions such as ALS.”

Yerres, Path Through the Old Growth Woods in the Park via Gustave Caillebotte

Size: 43x31 cm Medium: oil on canvas

The Poplar Avenue at Moret, Cloudy Day, Morning via Alfred Sisley

Size: 59x73 cm Medium: oil on canvas

Making twisted semiconductors for 3-D projection

A smartphone display that can produce 3-D images will need to be able to twist the light it emits. Now, researchers at the University of Michigan and the Ben-Gurion University of the Negev have discovered a way to mass-produce spiral semiconductors that can do just that.

Back in 1962, University of Michigan engineers E. Leith and J. Upatnieks unveiled realistic 3-D images with the invention of practical holography. The first holographic images of bird on a train were made by creating standing waves of light with bright and dark spots in space, which creates an illusion of material object. It was made possible by controlling polarization and phase of light, i.e. the direction and the timing of electromagnetic wave fluctuations.

The semiconductor helices created by U-M-led team can do exactly that with photons that pass through, reflected from, and emitted by them. They can be incorporated into other semiconductor devices to vary the polarization, phase, and color of light emitted by the different pixels, each of them made from the precisely designed semiconductor helices.

Read more.

Jeremy Miranda (American, b. 1980, Newport, RI, based Dover, NH, USA) - 1: Nectar, 2014, Oil on Panel  2: Waves Of Winter, 2014, Oil on Panel  3: Recording, 2014, Oil on Panel  4: Untitled, 2013  5: Know Your Garden, 2014, Oil on Panel  6: Renovation No. 2, Oil on Panel  7: Sea Foam, Oil on Panel  8: Salt Marsh, 2014  9: Untitled, 2014  10: Overgrown Path, 2013, Acrylics on Canvas

(Image caption: Brain showing hallmarks of Alzheimer’s disease (plaques in blue). Credit: ZEISS Microscopy)

New imaging technique measures toxicity of proteins associated with Alzheimer’s and Parkinson’s diseases

Researchers have developed a new imaging technique that makes it possible to study why proteins associated with Alzheimer’s and Parkinson’s diseases may go from harmless to toxic. The technique uses a technology called multi-dimensional super-resolution imaging that makes it possible to observe changes in the surfaces of individual protein molecules as they clump together. The tool may allow researchers to pinpoint how proteins misfold and eventually become toxic to nerve cells in the brain, which could aid in the development of treatments for these devastating diseases.

The researchers, from the University of Cambridge, have studied how a phenomenon called hydrophobicity (lack of affinity for water) in the proteins amyloid-beta and alpha synuclein – which are associated with Alzheimer’s and Parkinson’s respectively – changes as they stick together. It had been hypothesised that there was a link between the hydrophobicity and toxicity of these proteins, but this is the first time it has been possible to image hydrophobicity at such high resolution. Details are reported in the journal Nature Communications.

“These proteins start out in a relatively harmless form, but when they clump together, something important changes,” said Dr Steven Lee from Cambridge’s Department of Chemistry, the study’s senior author. “But using conventional imaging techniques, it hasn’t been possible to see what’s going on at the molecular level.”

In neurodegenerative diseases such as Alzheimer’s and Parkinson’s, naturally-occurring proteins fold into the wrong shape and clump together into filament-like structures known as amyloid fibrils and smaller, highly toxic clusters known as oligomers which are thought to damage or kill neurons, however the exact mechanism remains unknown.

For the past two decades, researchers have been attempting to develop treatments which stop the proliferation of these clusters in the brain, but before any such treatment can be developed, there first needs to be a precise understanding of how oligomers form and why.

“There’s something special about oligomers, and we want to know what it is,” said Lee. “We’ve developed new tools that will help us answer these questions.”

When using conventional microscopy techniques, physics makes it impossible to zoom in past a certain point. Essentially, there is an innate blurriness to light, so anything below a certain size will appear as a blurry blob when viewed through an optical microscope, simply because light waves spread when they are focused on such a tiny spot. Amyloid fibrils and oligomers are smaller than this limit so it’s very difficult to directly visualise what is going on.

However, new super-resolution techniques, which are 10 to 20 times better than optical microscopes, have allowed researchers to get around these limitations and view biological and chemical processes at the nanoscale.

Lee and his colleagues have taken super-resolution techniques one step further, and are now able to not only determine the location of a molecule, but also the environmental properties of single molecules simultaneously.

Using their technique, known as sPAINT (spectrally-resolved points accumulation for imaging in nanoscale topography), the researchers used a dye molecule to map the hydrophobicity of amyloid fibrils and oligomers implicated in neurodegenerative diseases. The sPAINT technique is easy to implement, only requiring the addition of a single transmission diffraction gradient onto a super-resolution microscope. According to the researchers, the ability to map hydrophobicity at the nanoscale could be used to understand other biological processes in future.