Penicillin

Cysteine Growth Requirements

MICROBIOLOGY MNEMONIC

BoyFriend Lost Penis

B rucella

F rancisella

L egionella

P asteurella

or….

The four sisters “Ella” worship in the “cystein” chapel

Brucella

Francisella

Legionella

Pasteurella

Most Common facts about infectious diseases

1. Most common cause of septic arthritis in a person less than 40 years old = Gonococcus 2. Most common cause of osteomyelitis in general population = S. aureus 3. Most common cause of osteomyelitis in Sickle Cell patients = Salmonella 4. Most common cause of osteomyelitis due to nail-puncture wounds = Pseudomonas (V.Imp!) 5. Most common parasitic infection of the brain = Neurocysticercosis 6. Most common cause of Encephalitis in USA = Herpes Simplex Virus (HSV) 7. Most common cause of dysentry in the USA = C. jejuni (undercooked poultry) 8. Second most cause of dysentry in the USA = Shigella (daycare centers) 9. Most common cause of pneumonia in nursing home residents = S. pneumoniae 10. Most common cause of malignant otitis externa = P. aeruginosa 11. Most common risk for contracting HIV in USA= Intravenous Drug Use 12. Most common presenting Manifestation of AIDs = P. carinii pneumonia 13. Most common cause of Menigitis in adolescents = N. Gonorhhaea 14. Most common cause of Meningitis in the USA = Streptococcus pneumoniae 15. Most common Neurological Manifestation of Lyme Disease = Facial Nerve palsy! 16. Most common Cardiac manifestation of Lyme Disease = AV Heart Block 17. Most Common viral STD in the USA = HPV ! 18. Most Common Complication of Mumps in Pre-pubertal Children = Encephalitis 19. Most Common Complication of Mumps in Pubertal and Post-Pubertal Adults (and Males) = Orchitis

Microbiology Genetics

Transformation

Conjugation:  F+ –> F-  &  Hfr –> F-

Transposition 

Transduction: Generalized   &  Specialized

ANTIBIOTICS CHEAT SHEET :)

Also, REMEMBER!!!!

* Sulfonamides compete for albumin with:

Bilirrubin: given in 2°,3°T, high risk or indirect hyperBb and kernicterus in premies

Warfarin: increases toxicity: bleeding

* Beta-lactamase (penicinillase) Suceptible:

Natural Penicillins (G, V, F, K)

Aminopenicillins (Amoxicillin, Ampicillin)

Antipseudomonal Penicillins (Ticarcillin, Piperacillin)

* Beta-lactamase (penicinillase) Resistant:

Oxacillin, Nafcillin, Dicloxacillin

3°G, 4°G Cephalosporins

Carbapenems 

Monobactams

Beta-lactamase inhibitors

* Penicillins enhanced with:

Clavulanic acid & Sulbactam (both are suicide inhibitors, they inhibit beta-lactamase)

Aminoglycosides (against enterococcus and psedomonas)

* Aminoglycosides enhanced with Aztreonam

* Penicillins: renal clearance EXCEPT Oxacillin & Nafcillin (bile)

* Cephalosporines: renal clearance EXCEPT Cefoperazone & Cefrtriaxone (bile)

* Both inhibited by Probenecid during tubular secretion.

* 2°G Cephalosporines: none cross BBB except Cefuroxime

* 3°G Cephalosporines: all cross BBB except Cefoperazone bc is highly highly lipid soluble, so is protein bound in plasma, therefore it doesn’t cross BBB.

* Cephalosporines are "LAME“ bc they  do not cover this organisms 

L  isteria monocytogenes

A  typicals (Mycoplasma, Chlamydia)

M RSA (except Ceftaroline, 5°G)

E  nterococci

* Disulfiram-like effect: Cefotetan & Cefoperazone (mnemonic)

* Cefoperanzone: all the exceptions!!!

All 3°G cephalosporins cross the BBB except Cefoperazone.

All cephalosporins are renal cleared, except Cefoperazone.

Disulfiram-like effect

* Against Pseudomonas:

3°G Cef taz idime (taz taz taz taz)

4°G Cefepime, Cefpirome (not available in the USA)

Antipseudomonal penicillins

Aminoglycosides (synergy with beta-lactams)

Aztreonam (pseudomonal sepsis)

* Covers MRSA: Ceftaroline (rhymes w/ Caroline, Caroline the 5°G Ceph), Vancomycin, Daptomycin, Linezolid, Tigecycline.

* Covers VRSA: Linezolid, Dalfopristin/Quinupristin

* Aminoglycosides: decrease release of ACh in synapse and act as a Neuromuscular blocker, this is why it enhances effects of muscle relaxants.

* DEMECLOCYCLINE: tetracycline that’s not used as an AB, it is used as tx of SIADH to cause Nephrogenic Diabetes Insipidus (inhibits the V2 receptor in collecting ducts)

* Phototoxicity: Q ue S T  ion?

Q uinolones

Sulfonamides

T etracyclines

* p450 inhibitors: Cloramphenicol, Macrolides (except Azithromycin), Sulfonamides

* Macrolides SE: Motilin stimulation, QT prolongation, reversible deafness, eosinophilia, cholestatic hepatitis

* Bactericidal: beta-lactams (penicillins, cephalosporins, monobactams, carbapenems), aminoglycosides, fluorquinolones, metronidazole.

* Baceriostatic: tetracyclins, streptogramins, chloramphenicol, lincosamides, oxazolidonones, macrolides, sulfonamides, DHFR inhibitors.

* Pseudomembranous colitis: Ampicillin, Amoxicillin, Clindamycin, Lincomycin.

* QT prolongation: macrolides, sometimes fluoroquinolones

fungal infectors by systems 

Sorry guys haven’t been updating much lately 

Another Harry Potter Mnemonic (yayyy!!!)

So, whenever I read about this fungi Sporothrix schenckii I think about Bellatrix Lestrange… geti it? 

Sporothrix - Bellatrix

That evil deatheater that killed Sirius Black. But they actually got it wrong in the movie, she doesn’t “Avada Kedavra” him, she just knocked him through that veil where you could hear death people….

Ok, enough with the Harry Potter fact.

To diagnose an infection from Sporothrix schenckii, you have to see this cigar shaped yeasts (from the pus of the lesions), which are the tissue form of the fungi. 

So to remember this, just picture:

“Bellatrix Lestrange smoking a cigar”

(Sporothrix: cigar shaped yeast in pus)

Actinomyces israelii

Gram+, anaerobic, non-spore forming, branching rod

Endogenous transmission (dental crevices -bad higiene, dental trauma- ; female genital tract -IUD-)

Dx: branching rods in “sulfur granules”; colonies resemble a molar tooth.

Not painful but very invasive penetrating tissues, including bone.

Draining abscess (sinus tracts) CULTURE THAT PUS

Disease: ACTINOMYCOSIS in low O2 tissues

Cervicofacil: “Lumpy jaw”, mycetoma on jaw line

Pelvic: from IUD

CNS: solitary abscess

Abdominal: qx, trauma

Thoracic: aspiration

Petechial hemorrhages on palms of hand in Rocky mountain spotted fever due to Rickettsia rickettsii: In RMSF, the petechial rash begins on the palms and spreads to the trunk.

Listeria with tumbling motility (by Pathmass)

Listeria monocytogenes

Gram+, aerobic, motile rod, facultative intracellular, beta hemolytic on blood agar

Tumbling motility in broth (as seen in video)

Jet motility in cells by actin filament formation

Listeriolysin O (Beta hemolysin): pathogenic factor, facilitates it scape from phagosome before phagolysosome formation and “jets” into another cell.

Diseases:

Lysteriosis: asymptomatic or diarrhea. 

Lysteriosis in pregnant women septicimia, crosses placenta.

Granulomatosis infantisepticum: neonatal disease, in utero transmission, sepsis, diseminated granulomas and high mortality.

Neonatal sepsis and meningitis (3° most common cause) 2-3 weeks after birth (fecal exposure)

Septicemia and meningitis in immunocompromised pts

Meningitis in renal transplant pts, cancer pts