Csmsdust

What it looks like when neurons make connections and communicate with each other

Credit: slava__bobrov 

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Feeling like a pro in Atomic Force Microscopy

...to be honest I probably should really tell myself that at this point I really am the professional in this method. After 4 years of working with 2 different atomic force microscopes, now I started with a 3rd one, again a new type from a different company.

Only after 2 hours of training on the new machine, I could observe membranes of resistant bacteria all by myself. The membranes are the yellow pancakes sitting flat on the dark support. They are less than 8 nm high (0.000000008 m), as is visible in the blue and red profile lines. So it's super tricky to actually see them. Atomic force microscope touches the surface of my membranes and surrounding support with a tiny tip like with a finger and reconstructs the surface topology. On top of the small size, the cellular membranes are super soft so also the touching finger must be super soft to see them without damaging them.

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Today I found a good video about merging tubes with different angles, ellipses, phase shift of sine waves, featuring sculptures by Frank Smullin.

This video is exceptionally comprehensive.

[Shared by hardm.ix on instagram: Text says: "A little more on the analytic constructivist sculpture of Frank Smullin, a professor of mine at Duke University who combined art and engineering in a way that reminded me a little of Kenneth Snelson and Tensegrity or Buckminster Fuller and geodesic domes."]

Walnut shells cut in half.

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Special brain cells react to unexpected situations

New research from the Netherlands Institute for Neuroscience shows that chandelier cells, a specific type of brain cell, become active during unexpected situations. “Researchers have been wondering about the functionality of these cells for a long time”.

You’re cycling to work through the city and suddenly you see a new building somewhere. On the first day that is very surprising. On day 2 this diminishes somewhat, and after a week you no longer notice it at all. The same thing happens the other way around: when a building that was always there suddenly disappeared, you are also surprised. But how does your brain signal unexpected changes and which cells are involved?

To learn more about this phenomenon, Koen Seignette from Christiaan Levelt’s lab joined forces with his colleagues from the Kole lab and Roelfsema lab. Together, they investigated a special type of brain cell found in small numbers in the cortex: the chandelier cell. In contrast to other inhibitory brain cells, they only inhibit one spot of other cells, but there is remarkably little known about why and when.

New mouse model

Koen Seignette: ‘We already knew quite a lot about the function of most types of inhibitory brain cells, but chandelier cells were a mystery. This is because they are not clearly marked genetically, and so could not be properly examined. We have now obtained a mouse model in which the chandelier cells are fluorescently labeled. This allows us to image them live and determine when they are active. That offers new opportunities.’

‘As a first step, we looked at what chandelier cells in the visual cortex respond to. What happens to these cells when the mouse starts running or when we present visual stimuli? In one of the experiments we had the mice walk in a virtual tunnel. When the mouse ran, the tunnel moved, and when it stopped, so did the tunnel. Using this setup, we could create an unexpected situation by stopping the tunnel while the mouse was still running. It was during these events that the chandelier cells started firing like crazy.’

Plasticity

Christiaan Levelt: ‘We see that the type of stimulus does not actually matter that much, what matters is that it is unexpected and surprising. We also noticed that habituation and change occurs, comparable to the aforementioned example of the new building. At first the cells react strongly, but after repeated exposure the activity becomes weaker. This shows that the cells are able to adapt, which is a concept known as plasticity. This plasticity also occurs at a structural anatomical level: we can literally see changes in the synapses chandelier cells form on other brain cells.’

‘What makes this study important is that this is the first really comprehensive study of chandelier cells in the visual cortex. We have not only determined what they respond to, but also which brain cells they form connections with, and what their influence is on other brain cells. This has never been looked at in such detail before. Understanding the role of these inhibitory neurons in the cortex is crucial for many processes, including learning from unexpected circumstances. We all know that you remember things better when it really surprises you. If the prediction is incorrect, that’s where you can find the information. You need plasticity to update your insights, and these cells could play a role in that.’

Why are chandelier cells so special?

Chandelier cells, named for their resemblance to a chandelier, are inhibitory brain cells that focus on the starting point (axon initial segment) of electrical signals in the pyramidal cells, the most common cells in the cortex. It was thought that chandelier cells could exert strong control over pyramidal cells by blocking the action potential. Surprisingly, the current research shows that this effect is actually very weak, which contradicts previously drawn conclusions.