Not Now, Im Noticing The Pattern

Birthday presents ✨️

@academia-lucifer

The Myth of Dreamless Sleep

Consciousness never "switches off" because it isn't generated by the brain. Its sensory inputs can be switched off - during sleep or general anesthesia, for instance - but your consciousness is still there. For example, a small but consistent number of patients report out-of-body experiences (OOBEs) or near-death experiences (NDEs) while under general anesthesia. As someone with REM Sleep Behavior Disorder (RBD) I can attest to this, since I act out dreams that occur during the non-REM phases of sleep.

ScienceAlert

We Were Wrong About Consciousness Disappearing in Dreamless Sleep, Say Scientists

When it comes to dreamlessness, conventional wisdom states that consciousness disappears when we fall into a deep, dreamless sleep.  But res

Feeling like a pro in Atomic Force Microscopy

...to be honest I probably should really tell myself that at this point I really am the professional in this method. After 4 years of working with 2 different atomic force microscopes, now I started with a 3rd one, again a new type from a different company.

Only after 2 hours of training on the new machine, I could observe membranes of resistant bacteria all by myself. The membranes are the yellow pancakes sitting flat on the dark support. They are less than 8 nm high (0.000000008 m), as is visible in the blue and red profile lines. So it's super tricky to actually see them. Atomic force microscope touches the surface of my membranes and surrounding support with a tiny tip like with a finger and reconstructs the surface topology. On top of the small size, the cellular membranes are super soft so also the touching finger must be super soft to see them without damaging them.

shit man this got me emotional

Work on a scientific article

What it actuallly entails:

Come up with an idea, define an interesting problem

Do thorough literature research. Maybe similar stuff was already done. Define the knowledge gap well.

Plan in detail, how we can solve the problem, design experiments

Reach out to potential collaborators, agree with them on a plan

Buy necessary equipment, chemicals

Do pilot experiment, optimize the conditions to get reliable data

Perform experiments, calculations, make everything multiple times so it's reliable

Analyze the data

Urge collaborators to deliver their parts

Coordinate your progress with the collaborators

Manage the collaborations, organize meetings

Be diplomatic, you don't want to make enemies in academia

Agree with direct colleagues, who worked on it, what will be the message of the article. Will it be a long story and we need to add some more data? Or will it be short and right to the point and we write a short "letter"?

Do literature research again. Maybe new stuff appeared, and for sure your data must be confronted and discussed with already known facts.

Write the first draft of the article

Send it around for feedback, first only to direct colleagues from your lab

Incorporate the feedback, maybe do more experiments and more analysis

Rewrite the manuscript

Send it around the second, third, fourth, fifth... time

Incorporate the feedback

Send the manuscript to all collaborators.

Wait for the feedback, urge everyone to give it, maybe you don't have all data from all the collaborators yet

Incorporate feedback

Prepare the manuscript for journal submission

Get approval from all co-authors

Submit the manuscript

Wait for editor response, hopefully they send it to reviewers. If not, you need to rewrite a bit the article to adhere to the new journal's format and send somewhere else.

Get reviewers' reports, deal with them, reply truthfully, make effort to explain everything even if you know that the reviewer's suggestion is just impossible or irrelevant. Be diplomatic.

Maybe you need to do an additional experiment, analysis, or rewrite a major part fo the manuscript. This can take months.

Submit revised manuscript with all the changes

Wait for editor's nad reviewers' comments in the second round. You can get many rounds of review and still get rejected.

Finally get a "Congratulations, your manuscript has been accepted for publication"

Pop a shampagne! You deserve it!

What part of this do you usually do in different career stages:

BSc. and MSc. students: Perform experiments and analyze data

PhD students: Do all the experimental and analysis parts, write the manuscript, discuss with their supervisor and direct colleagues, incorporate feedback. But does not have to come up with their own idea and manage collaborations and diplomacy.

Postdocs: Do literally everything on the list

Group leader/Professor: Do the thinking and managing parts, help with writing and feedback, provide discussions and insight. Do not perform actual experiments and analysis.

Being a postdoc is the transformation between the student and the group leader.

As such, we just have to do all these tasks. It's stressful. It's challenging. It's definitely not boring. I am taking every opportunity to get a student, who can help with the experimental repetitions so I have time for all the other stuff.

Cosmic clouds form fantastic shapes in the central regions of emission nebula IC 1805. The clouds are sculpted by stellar winds and radiation from massive hot stars in the nebula's newborn star cluster, Melotte 15. IC 1805 is located about 7,500 light years away toward the boastful constellation Cassiopeia.

Image Credit: Richard McInnis